In April 2024, the company’s two patent applications, “Enzymes and Their Applications in the Preparation of Non-Natural Amino Acid Dipeptides” and “β-Alanine Ligase Mutants and Their Applications,” were granted national invention patents. This achievement marks the world’s first large-scale enzymatic production of carnosine. The receipt of these two national invention patents signifies that the company’s green carnosine synthesis process and technological innovation capabilities have received authoritative national recognition!
1. Enzymes and their application in the preparation of non-natural amino acid dipeptides
“This invention relates to the field of bioengineering technology, and more particularly to enzymes and their applications in the preparation of non-natural amino acid dipeptides. This invention utilizes a protein derived from Staphylococcus aureus as a template for modification, and through mutation of its catalytically active pockets and other related amino acid residues, ultimately obtains a highly catalytically active enzyme for producing carnosine.”.
2. β-Alanine ligase mutants and their applications
“This invention relates to the field of biotechnology, and particularly to β-alanine ligase mutants and their applications. This invention utilizes the characteristic of an L-amino acid ligase in Actinobacteria that can catalyze the linkage of β-alanine with other amino acids. By mutating the catalytically active pockets and other related amino acid residues, highly catalytically active carnosine is ultimately obtained. This method is significantly superior to chemical synthesis processes, exhibiting outstanding advantages in production cost, energy consumption, product quality, and green index. Therefore, large-scale production using this method will be the preferred option for carnosine production.”
1. Advanced Skincare Solution with Efficacy – Green Carnosine
L-Carnosine, scientifically known as β-alanyl-L-histidineCarnosine is a naturally occurring dipeptide found in human tissues. It is renowned for its excellent antioxidant and anti-glycation effects, effectively scavenging free radicals and protecting mitochondria from oxidative damage.Simultaneously, it competitively prevents the cross-linking reaction between proteins and reducing sugars, thus inhibiting the formation of AGEs.
Mechanism of action of carnosine “dual resistance”
Carnosine is generally synthesized chemically, and the product is prone to residual hydrazine hydrate, which affects its application in cosmetics. As a pioneer in peptide biosynthesis, Enzyme Products, based on a leading green bio-manufacturing platform in China, has achieved the world’s first large-scale production of carnosine using enzyme-directed evolution technology. The product has a purity of up to 99.9%, is free of hydrazine hydrate, and is 66.7% bio-based.
Comparison of carnosine production methods
The enzyme-based green carnosine has received COSMOS Approved certification, demonstrating excellent safety, stability, and transdermal absorption. Furthermore, experimental data indicates that compared to similar carnosine products, the enzyme-based green carnosine exhibits stronger anti-glycation and anti-inflammatory effects .
COSMOS APPROVED Certification
Comparative experimental data on the efficacy of carnosine products
All enzyme products sold have been tested by professional laboratories, ensuring reliable quality and stable production capacity. Complete safety assessment reports are available, giving you peace of mind with your formulations and accelerating new product development!
2. Regarding finished enzyme products
Enzyme Products is committed to becoming a global leader in next-generation green active ingredients. Utilizing next-generation synthetic biology technology, the company focuses on the green production of active ingredients such as peptides, proteins, sugars, and nucleic acids. Leveraging over 20 years of research and industry experience accumulated by its founding team, the company has established a leading multidisciplinary technology platform in China. It has now achieved mass production of dozens of green active ingredients, including S- Proxine, copper peptide, ergothioneine, NMN, acetyl hexapeptide-8, and smegglutinin , providing high-quality green active ingredients to nearly a thousand well-known companies in the fields of functional skincare, biomedicine, and nutritional health .
After several years of rapid development, the company has achieved a leading position among domestic enterprises in terms of R&D investment, scale, and output. It has applied for more than 100 invention patents and has won more than ten national and local titles, including National Specialized and Innovative Small Giant, National High-tech Enterprise, Shenzhen Specialized and Innovative Small and Medium Enterprise, Shenzhen Potential Unicorn Enterprise, Shenzhen Postdoctoral Innovation Practice Base, Shenzhen Top 30 Medical and Health Innovation Team, Zhuhai Innovation and Entrepreneurship Team, and Gansu Specialized and Innovative Small and Medium Enterprise. In 2022, it was selected as one of Shenzhen’s Top 100 High-Growth Enterprises (first among unlisted biotech companies), and in 2023, it was selected as a Harvard Business Review High-Energy Innovation Team.